Error message upon launching the server: ?

1
2
3

sudo mkdir /sys/fs/cgroup/systemd
sudo mount -t cgroup -o none,name=systemd cgroup /sys/fs/cgroup/systemd

Error message when loading a page from AWS:

LIMS*Nucleus falls into the “systems” category of LIMS development. LIMS*Nucleus contains a limited set of features and is designed to be integrated into a larger system.

Goal

Comprehensive but limitted feature set (multi-well plate sample management) with well defined inputs and outputs designed to be incorporated into a larger system.

Implemented features

Create project, plate set, plates, wells with and without samples
Group plate sets into a new plate set
Subset plates from a plate set into a new plate set
Reformat plates from 96 into 384 well plates and 384 into 1536 well plates
Apply assay data to plate sets
Visually or algoritnmlically evaluate assay data
Identify hits as samples surpassing a threshold; create hit lists
Create worklists for liquid handling robots
Rearray hits from a hit list into a new plate set
Apply user generated barcodes to plates
Apply user generated accession IDs to samples

Next>> Canonical workflow

LIMS*Nucleus defines a hierarchy of 5 entities.

A project contains plate sets, each of which contains plates etc. Projects can only be created by an administrator. Plate Sets can be created by users. Other entities are created automatically as needed. Plates, wells, samples are created at the time of plate set creation.
Plates and wells are created automatically during rearraying operations.

Once the license key is available, navigate to Utilities/Register menu item in the software and enter customer ID, license key and your email. These will be registered in a local database. This is a one time process.

Click ‘Register’ to update the local database and confirm validity of the license key.

Problems?: info@labsolns.com Note that internet access is required for the licensing mechanism to work. If Blockchain.info is down, please try again at a later time.

Configuration of LIMS*Nucleus is achieved by editing either /etc/artanis/artanis.conf or $HOME/.config/limsn/artanis.conf, depending on how LIMS*Nucleus was installed. LIMS*Nucleus specific parameters are listed below:

Database

These parameter will need to be edited if you utilize a cloud provider. These are the parameters that comprise the connection string. PostgreSQL is the only database with which LIMS*Nucleus is compatible.

Web Server

LIMS*Nucleus specific

• Note that the ‘maxplates’ parameter is in the cookie group only for convenience. A maxplates cookie is never set. The only cookie utilized by LIMS*Nucleus is the session id ‘sid’.

1. Click the ‘Projects’ hyperlink on the global navigation pane. Navigate into the project that will contain the plate set. Note that the ‘Plate Set For…’ link now indicates the default working project.

2. Select the check box near the plate set into which you would like to add a new plate set. Click the tools icon and select add to project…

3. Fill in the required information and click submit.

Available layouts are presented in the dropdown appropriate to the plate format/type:

Once complete, the new plate set will be visible in the client window.

Email already exists.
Wallet/Customer ID not generated.

Contact info@labsolns.com for assistance.

Barcode IDs are imported by plate number. The files page provides details on the file format, with a 10 plate sample file available. Navigate into the project of interest and highlight the plate set of interest. From the menu select Utilities/Import Barcodes:

The import barcode form will appear - select file. A truncated sample of file contents will appear belwo the ‘Submit’ button for confirmation:

The example barcode import file looks like:

1
2
3
4
5
6
7
8
9
10
11

plate 	barcode.id
1	LN000001
2	LN000002
3	LN000003
4	LN000004
5	LN000005
6	LN000006
7	LN000007
8	LN000008
9	LN000009
10	LN000010

Once imported, barcode ids will appear in the plate table:

Example barcode ID import file:

Below is the canonical workflow that LIMS*Nucleus is designed to handle.

1. Create a plate set.
   1.5 Possibly reformat the plate set into higher density plates for assay.
2. Create assay plates and run an assay. Apply assay data to the assay plate set.
3. Identify hits
4. Rearray hits into a new plate set
5. (Reformat hits into higher density plates), run hits in a secondary assay, identify hits, rearray, etc.

Tutorial 1 will walk you through this workflow.

Next>> Evaluate

Thank you for your request.

An invoice in the amount of $5000 for a LIMS*Nucleus license key will be emailed to:

info@labsolns.com

Table of Contents