LIMS*Nucleus example database contains plate sets, plates, hit list, assay runs and data. The various projects are at different stages of completion to demonstrate the functionality of the application.
| Project ID | Description | Utility |
|---|---|---|
| PRJ-1 | 3 plate sets with 2 96 well plates each; includes data and hit lists | visualize data; visualize hit lists; group plate sets; |
| PRJ-2 | 1 plate set with 2 384 well plates each; includes data | create hit lists |
| PRJ-3 | 1 plate set with 1 1536 well plate | visualize data |
| PRJ-10 | 2 plate sets with 10 96 well plates each | add data;create hit lists; subselect plate sets |
Files for import
Provide your own import files or use the supplied data below:
| Description | File |
|---|---|
| Accession IDSs for PS-1 ( 2 96 well plates, 4 controls) | accs96x2controls4.txt |
| ELISA data for 2 96 well plates with 4 controls col 12 (LYT-1) | sample96controls4lowp2.txt |
| HTRF data for 5 96 well plates with 8 controls col 12 (LYT-7) | sample96controls8low5plates.txt |
| A new 384 well layout with controls randomly placed | controls8scatteredNoEdge384.txt |
Statistics for example assay data files
|Assay Run|File|Response|mean(neg)|stdev(neg)|neg + 3SD|neg hits|mean(pos)|pos hits|
|–|–|–|–|–|–|
|AR-1|ar1data.txt|raw|0.1155|0.07|0.3255|53|0.586|7|
|||bkgrnd_sub|0.1146|0.0697|0.3236|53|0.5852|7|
|||norm|0.1807|0.1172|0.5322|48|0.9076|6|
|||norm_pos|0.1971|0.1236|0.568|51|0.9985|7|
|||% enhanced|NA|0|NA|NA|0|7|
|AR-2|ar2data.txt|raw|0.0945|0.0332|0.1942|125|0.567|6|
|||bkgrnd_sub|0.0922|0.0357|0.1993|117|0.5646|6|
|||norm|0.1381|0.0601|0.3184|115|0.8371|6|
|||norm_pos|0.1641|0.0703|0.3751|115|0.9959|6|
|||% enhanced|NA|NA|NA|NA|0|6|
|AR-3|ar3data.txt|raw|2850|2616.2951|10698.8853|89|24200|3|
|||bkgrnd_sub|2849.9978|2616.2935|10698.8781|89|24199.997|3|
|||norm|0.1102|0.1076|0.4331|78|0.8908|4|
|||norm_pos|0.1184|0.1095|0.4469|88|1|3|
|||% enhanced|NA|NA|NA|NA|0|3|
|AR-4|ar4data.txt|raw|0.109|0.066|0.3069|288|0.5649|27|
|||bkgrnd_sub|0.1076|0.066|0.3057|287|0.5635|27|
|||norm|0.1513|0.0956|0.438|291|0.794|36|
|||norm_pos|0.1906|0.1168|0.5411|288|0.9976|27|
|||% enhanced|NA|NA|NA|NA|0|27|
|AR-5|ar5data.txt|raw|0.0764|0.0512|0.23|857|0.5787|48|
|||bkgrnd_sub|0.0728|0.0512|0.2265|857|0.5752|48|
|||norm|0.0895|0.0629|0.2783|857|0.7067|48|
|||norm_pos|0.1259|0.0885|0.3914|857|0.9939|48|
|||% enhanced|NA|NA|NA|NA|0|48|